Exogenous GbHMGS1 Overexpression Improves the Contents of Three Terpenoids in Transgenic Populus

Ginkgo biloba L. has attracted much attention due to its medicinal properties, particularly those of terpenoid and flavonoid contents. However, the content utilization efficiency terpenoids remain low. The enzyme 3-hydroxy-3-methylglutaryl CoA synthase (HMGS) is a major rate-limiting factor, RNA-seq revealed that mRNA expression this differentially expressed during biosynthesis. Here, we investigated function GbHMGS1 gene overexpression in Populus. We compared metabolite contents nontransgenic (CK) Populus with transgenic lines through metabolomics analysis. Our results indicate protein localized cytoplasm. Significant differences chemical characteristics were found between CK plants, total 31 metabolites upregulated plants. also higher lanosterol (triterpenoid), dehydroabietic acid (diterpenoid), phytol (diterpenoid) plants than their counterparts. thus speculate might regulate plant-related product formation increase This study molecular mechanism governing synthesis suggested one triterpenoid two diterpenoids significant upregulation can be used as markers for breeding specific metabolism-related characteristics.